ns hu 201642 tissue microarray anti sp1 (Cell Signaling Technology Inc)
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ns hu 201642 tissue microarray anti sp1
Ns Hu 201642 Tissue Microarray Anti Sp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ns+hu+201642+tissue+microarray+anti+sp1/IL2-Ralpha%2FCD25+Mouse+mAb/pmc08975516-247-143-149
Average 93 stars, based on 15 article reviews
Ns Hu 201642 Tissue Microarray Anti Sp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ns+hu+201642+tissue+microarray+anti+sp1/IL2-Ralpha%2FCD25+Mouse+mAb/pmc08975516-247-143-149
Average 93 stars, based on 15 article reviews
ns hu 201642 tissue microarray anti sp1 - by Bioz Stars,
2026-09
93/100 stars
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Staining:Article Title: Prognostic Value of COX-2, NF-κB, and Sp1 Tissue Expressions in Pancreatic Ductal Adenocarcinoma: A Systematic Review and Meta-analysis Article Snippet: General Study Characteristics table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Study Pathologic Specimen Primary Antibody Detection Method Scoring System Controls Fagman 201923 Whole section Polyclonal rabbit antiCOX2 (cytosolic detection), ab15191, Abcam, Cambridge, UK, dilution 1 : 200 Biotin-free polymer-based - COX-2 high grade = >median score Negative control = sections stained by mouse IgG1 (X0931, Agilent Technologies) and rabbit IgG1 (X0903, Agilent Technologies) diluted in 5% non-fat milk and TBS to the same protein concentration as the primary antibody - Score = average intensity score x average percentage of tumor cells showing positive staining Juuti 20065 Whole section Mouse antihuman anti-COX-2 monoclonal antibody (160112;5 Cayman Chemical, Ann Arbor, Michigan, USA) dilution 1 : 200 Avidin–biotin–peroxidase system COX-2 (+) = >5% cells stained with moderate intensity - External positive control: a colon sample containing adenocarcinoma cells stained >50%, and adjacent epithelial non-neoplastic cells stained 5-0% - Internal positive control: pancreatic islet cells that consistently expressed COX-2 - Negative control: Sections with phosphate buffered saline or nonimmune antibody Matsubayashi 20076 Tissue Microarray Anti-COX-2 monoclonal antibody (Cayman Chemical, Ann Arbor, MI) dilution 1 : 100 NS COX-2 (+) = staining intensity was moderate (++) to strong (+++) and the extent was ≥10% in the neighboring fibroblasts NS Pomianowska 201413 Whole Section Monoclonal anti-COX-2 antibodies (Thermo Fischer Scientific rabbit), dilution not specified Biotin-free polymer-based - COX-2 (+) = immunoscore >1.4 - Positive control = Islets of Langerhans and duodenum mucosa - Negative control = identical sections without the primary antibody - Immunoscore = the average numeric scores of 5 high-power fields from each case to be considered positive in intensity scoring. .. - 1 = < 10% cells stained, 2 = 10-50% and 3 > 50% Schmid 201340 Whole section Mouse monoclonal anti-COX-2 antibody (clone CX220; Cayman Chemical, Ann Arbor, Mich) dilution 1 : 200 Streptavidin–biotin–peroxidase system - COX-2 (+) = detectable staining - Negative Control: incubating the sections without primary antibody - Internal positive control: immunoreactivity of the pancreatic islets Tonini 200541 Whole section Goat polyclonal anti-COX-2 (Santa Cruz Biotechnologies, Santa Cruz, CA, USA), dilution not specified Streptavidin–biotin–peroxidase system - COX-2 (+) = score ≥+1 (positive staining) - Negative control: sections with rabbit or goat preimmune serum Hang 201629 Tissue Microarray Anti-COX-2 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified NS - COX-2 or Sp1 (+) = score ≥ 4 NS - Score = staining intensity (0-3) x % of cells stained (1-4) Anti-Sp1 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified Negative Control:Article Title: Prognostic Value of COX-2, NF-κB, and Sp1 Tissue Expressions in Pancreatic Ductal Adenocarcinoma: A Systematic Review and Meta-analysis Article Snippet: General Study Characteristics table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Study Pathologic Specimen Primary Antibody Detection Method Scoring System Controls Fagman 201923 Whole section Polyclonal rabbit antiCOX2 (cytosolic detection), ab15191, Abcam, Cambridge, UK, dilution 1 : 200 Biotin-free polymer-based - COX-2 high grade = >median score Negative control = sections stained by mouse IgG1 (X0931, Agilent Technologies) and rabbit IgG1 (X0903, Agilent Technologies) diluted in 5% non-fat milk and TBS to the same protein concentration as the primary antibody - Score = average intensity score x average percentage of tumor cells showing positive staining Juuti 20065 Whole section Mouse antihuman anti-COX-2 monoclonal antibody (160112;5 Cayman Chemical, Ann Arbor, Michigan, USA) dilution 1 : 200 Avidin–biotin–peroxidase system COX-2 (+) = >5% cells stained with moderate intensity - External positive control: a colon sample containing adenocarcinoma cells stained >50%, and adjacent epithelial non-neoplastic cells stained 5-0% - Internal positive control: pancreatic islet cells that consistently expressed COX-2 - Negative control: Sections with phosphate buffered saline or nonimmune antibody Matsubayashi 20076 Tissue Microarray Anti-COX-2 monoclonal antibody (Cayman Chemical, Ann Arbor, MI) dilution 1 : 100 NS COX-2 (+) = staining intensity was moderate (++) to strong (+++) and the extent was ≥10% in the neighboring fibroblasts NS Pomianowska 201413 Whole Section Monoclonal anti-COX-2 antibodies (Thermo Fischer Scientific rabbit), dilution not specified Biotin-free polymer-based - COX-2 (+) = immunoscore >1.4 - Positive control = Islets of Langerhans and duodenum mucosa - Negative control = identical sections without the primary antibody - Immunoscore = the average numeric scores of 5 high-power fields from each case to be considered positive in intensity scoring. .. - 1 = < 10% cells stained, 2 = 10-50% and 3 > 50% Schmid 201340 Whole section Mouse monoclonal anti-COX-2 antibody (clone CX220; Cayman Chemical, Ann Arbor, Mich) dilution 1 : 200 Streptavidin–biotin–peroxidase system - COX-2 (+) = detectable staining - Negative Control: incubating the sections without primary antibody - Internal positive control: immunoreactivity of the pancreatic islets Tonini 200541 Whole section Goat polyclonal anti-COX-2 (Santa Cruz Biotechnologies, Santa Cruz, CA, USA), dilution not specified Streptavidin–biotin–peroxidase system - COX-2 (+) = score ≥+1 (positive staining) - Negative control: sections with rabbit or goat preimmune serum Hang 201629 Tissue Microarray Anti-COX-2 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified NS - COX-2 or Sp1 (+) = score ≥ 4 NS - Score = staining intensity (0-3) x % of cells stained (1-4) Anti-Sp1 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified Positive Control:Article Title: Prognostic Value of COX-2, NF-κB, and Sp1 Tissue Expressions in Pancreatic Ductal Adenocarcinoma: A Systematic Review and Meta-analysis Article Snippet: General Study Characteristics table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Study Pathologic Specimen Primary Antibody Detection Method Scoring System Controls Fagman 201923 Whole section Polyclonal rabbit antiCOX2 (cytosolic detection), ab15191, Abcam, Cambridge, UK, dilution 1 : 200 Biotin-free polymer-based - COX-2 high grade = >median score Negative control = sections stained by mouse IgG1 (X0931, Agilent Technologies) and rabbit IgG1 (X0903, Agilent Technologies) diluted in 5% non-fat milk and TBS to the same protein concentration as the primary antibody - Score = average intensity score x average percentage of tumor cells showing positive staining Juuti 20065 Whole section Mouse antihuman anti-COX-2 monoclonal antibody (160112;5 Cayman Chemical, Ann Arbor, Michigan, USA) dilution 1 : 200 Avidin–biotin–peroxidase system COX-2 (+) = >5% cells stained with moderate intensity - External positive control: a colon sample containing adenocarcinoma cells stained >50%, and adjacent epithelial non-neoplastic cells stained 5-0% - Internal positive control: pancreatic islet cells that consistently expressed COX-2 - Negative control: Sections with phosphate buffered saline or nonimmune antibody Matsubayashi 20076 Tissue Microarray Anti-COX-2 monoclonal antibody (Cayman Chemical, Ann Arbor, MI) dilution 1 : 100 NS COX-2 (+) = staining intensity was moderate (++) to strong (+++) and the extent was ≥10% in the neighboring fibroblasts NS Pomianowska 201413 Whole Section Monoclonal anti-COX-2 antibodies (Thermo Fischer Scientific rabbit), dilution not specified Biotin-free polymer-based - COX-2 (+) = immunoscore >1.4 - Positive control = Islets of Langerhans and duodenum mucosa - Negative control = identical sections without the primary antibody - Immunoscore = the average numeric scores of 5 high-power fields from each case to be considered positive in intensity scoring. .. - 1 = < 10% cells stained, 2 = 10-50% and 3 > 50% Schmid 201340 Whole section Mouse monoclonal anti-COX-2 antibody (clone CX220; Cayman Chemical, Ann Arbor, Mich) dilution 1 : 200 Streptavidin–biotin–peroxidase system - COX-2 (+) = detectable staining - Negative Control: incubating the sections without primary antibody - Internal positive control: immunoreactivity of the pancreatic islets Tonini 200541 Whole section Goat polyclonal anti-COX-2 (Santa Cruz Biotechnologies, Santa Cruz, CA, USA), dilution not specified Streptavidin–biotin–peroxidase system - COX-2 (+) = score ≥+1 (positive staining) - Negative control: sections with rabbit or goat preimmune serum Hang 201629 Tissue Microarray Anti-COX-2 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified NS - COX-2 or Sp1 (+) = score ≥ 4 NS - Score = staining intensity (0-3) x % of cells stained (1-4) Anti-Sp1 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified Microarray:Article Title: Prognostic Value of COX-2, NF-κB, and Sp1 Tissue Expressions in Pancreatic Ductal Adenocarcinoma: A Systematic Review and Meta-analysis Article Snippet: General Study Characteristics table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Study Pathologic Specimen Primary Antibody Detection Method Scoring System Controls Fagman 201923 Whole section Polyclonal rabbit antiCOX2 (cytosolic detection), ab15191, Abcam, Cambridge, UK, dilution 1 : 200 Biotin-free polymer-based - COX-2 high grade = >median score Negative control = sections stained by mouse IgG1 (X0931, Agilent Technologies) and rabbit IgG1 (X0903, Agilent Technologies) diluted in 5% non-fat milk and TBS to the same protein concentration as the primary antibody - Score = average intensity score x average percentage of tumor cells showing positive staining Juuti 20065 Whole section Mouse antihuman anti-COX-2 monoclonal antibody (160112;5 Cayman Chemical, Ann Arbor, Michigan, USA) dilution 1 : 200 Avidin–biotin–peroxidase system COX-2 (+) = >5% cells stained with moderate intensity - External positive control: a colon sample containing adenocarcinoma cells stained >50%, and adjacent epithelial non-neoplastic cells stained 5-0% - Internal positive control: pancreatic islet cells that consistently expressed COX-2 - Negative control: Sections with phosphate buffered saline or nonimmune antibody Matsubayashi 20076 Tissue Microarray Anti-COX-2 monoclonal antibody (Cayman Chemical, Ann Arbor, MI) dilution 1 : 100 NS COX-2 (+) = staining intensity was moderate (++) to strong (+++) and the extent was ≥10% in the neighboring fibroblasts NS Pomianowska 201413 Whole Section Monoclonal anti-COX-2 antibodies (Thermo Fischer Scientific rabbit), dilution not specified Biotin-free polymer-based - COX-2 (+) = immunoscore >1.4 - Positive control = Islets of Langerhans and duodenum mucosa - Negative control = identical sections without the primary antibody - Immunoscore = the average numeric scores of 5 high-power fields from each case to be considered positive in intensity scoring. .. - 1 = < 10% cells stained, 2 = 10-50% and 3 > 50% Schmid 201340 Whole section Mouse monoclonal anti-COX-2 antibody (clone CX220; Cayman Chemical, Ann Arbor, Mich) dilution 1 : 200 Streptavidin–biotin–peroxidase system - COX-2 (+) = detectable staining - Negative Control: incubating the sections without primary antibody - Internal positive control: immunoreactivity of the pancreatic islets Tonini 200541 Whole section Goat polyclonal anti-COX-2 (Santa Cruz Biotechnologies, Santa Cruz, CA, USA), dilution not specified Streptavidin–biotin–peroxidase system - COX-2 (+) = score ≥+1 (positive staining) - Negative control: sections with rabbit or goat preimmune serum Hang 201629 Tissue Microarray Anti-COX-2 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified NS - COX-2 or Sp1 (+) = score ≥ 4 NS - Score = staining intensity (0-3) x % of cells stained (1-4) Anti-Sp1 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified Polymer:Article Title: Prognostic Value of COX-2, NF-κB, and Sp1 Tissue Expressions in Pancreatic Ductal Adenocarcinoma: A Systematic Review and Meta-analysis Article Snippet: General Study Characteristics table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Study Pathologic Specimen Primary Antibody Detection Method Scoring System Controls Fagman 201923 Whole section Polyclonal rabbit antiCOX2 (cytosolic detection), ab15191, Abcam, Cambridge, UK, dilution 1 : 200 Biotin-free polymer-based - COX-2 high grade = >median score Negative control = sections stained by mouse IgG1 (X0931, Agilent Technologies) and rabbit IgG1 (X0903, Agilent Technologies) diluted in 5% non-fat milk and TBS to the same protein concentration as the primary antibody - Score = average intensity score x average percentage of tumor cells showing positive staining Juuti 20065 Whole section Mouse antihuman anti-COX-2 monoclonal antibody (160112;5 Cayman Chemical, Ann Arbor, Michigan, USA) dilution 1 : 200 Avidin–biotin–peroxidase system COX-2 (+) = >5% cells stained with moderate intensity - External positive control: a colon sample containing adenocarcinoma cells stained >50%, and adjacent epithelial non-neoplastic cells stained 5-0% - Internal positive control: pancreatic islet cells that consistently expressed COX-2 - Negative control: Sections with phosphate buffered saline or nonimmune antibody Matsubayashi 20076 Tissue Microarray Anti-COX-2 monoclonal antibody (Cayman Chemical, Ann Arbor, MI) dilution 1 : 100 NS COX-2 (+) = staining intensity was moderate (++) to strong (+++) and the extent was ≥10% in the neighboring fibroblasts NS Pomianowska 201413 Whole Section Monoclonal anti-COX-2 antibodies (Thermo Fischer Scientific rabbit), dilution not specified Biotin-free polymer-based - COX-2 (+) = immunoscore >1.4 - Positive control = Islets of Langerhans and duodenum mucosa - Negative control = identical sections without the primary antibody - Immunoscore = the average numeric scores of 5 high-power fields from each case to be considered positive in intensity scoring. .. - 1 = < 10% cells stained, 2 = 10-50% and 3 > 50% Schmid 201340 Whole section Mouse monoclonal anti-COX-2 antibody (clone CX220; Cayman Chemical, Ann Arbor, Mich) dilution 1 : 200 Streptavidin–biotin–peroxidase system - COX-2 (+) = detectable staining - Negative Control: incubating the sections without primary antibody - Internal positive control: immunoreactivity of the pancreatic islets Tonini 200541 Whole section Goat polyclonal anti-COX-2 (Santa Cruz Biotechnologies, Santa Cruz, CA, USA), dilution not specified Streptavidin–biotin–peroxidase system - COX-2 (+) = score ≥+1 (positive staining) - Negative control: sections with rabbit or goat preimmune serum Hang 201629 Tissue Microarray Anti-COX-2 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified NS - COX-2 or Sp1 (+) = score ≥ 4 NS - Score = staining intensity (0-3) x % of cells stained (1-4) Anti-Sp1 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified Concentration Assay:Article Title: Prognostic Value of COX-2, NF-κB, and Sp1 Tissue Expressions in Pancreatic Ductal Adenocarcinoma: A Systematic Review and Meta-analysis Article Snippet: General Study Characteristics table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Study Pathologic Specimen Primary Antibody Detection Method Scoring System Controls Fagman 201923 Whole section Polyclonal rabbit antiCOX2 (cytosolic detection), ab15191, Abcam, Cambridge, UK, dilution 1 : 200 Biotin-free polymer-based - COX-2 high grade = >median score Negative control = sections stained by mouse IgG1 (X0931, Agilent Technologies) and rabbit IgG1 (X0903, Agilent Technologies) diluted in 5% non-fat milk and TBS to the same protein concentration as the primary antibody - Score = average intensity score x average percentage of tumor cells showing positive staining Juuti 20065 Whole section Mouse antihuman anti-COX-2 monoclonal antibody (160112;5 Cayman Chemical, Ann Arbor, Michigan, USA) dilution 1 : 200 Avidin–biotin–peroxidase system COX-2 (+) = >5% cells stained with moderate intensity - External positive control: a colon sample containing adenocarcinoma cells stained >50%, and adjacent epithelial non-neoplastic cells stained 5-0% - Internal positive control: pancreatic islet cells that consistently expressed COX-2 - Negative control: Sections with phosphate buffered saline or nonimmune antibody Matsubayashi 20076 Tissue Microarray Anti-COX-2 monoclonal antibody (Cayman Chemical, Ann Arbor, MI) dilution 1 : 100 NS COX-2 (+) = staining intensity was moderate (++) to strong (+++) and the extent was ≥10% in the neighboring fibroblasts NS Pomianowska 201413 Whole Section Monoclonal anti-COX-2 antibodies (Thermo Fischer Scientific rabbit), dilution not specified Biotin-free polymer-based - COX-2 (+) = immunoscore >1.4 - Positive control = Islets of Langerhans and duodenum mucosa - Negative control = identical sections without the primary antibody - Immunoscore = the average numeric scores of 5 high-power fields from each case to be considered positive in intensity scoring. .. - 1 = < 10% cells stained, 2 = 10-50% and 3 > 50% Schmid 201340 Whole section Mouse monoclonal anti-COX-2 antibody (clone CX220; Cayman Chemical, Ann Arbor, Mich) dilution 1 : 200 Streptavidin–biotin–peroxidase system - COX-2 (+) = detectable staining - Negative Control: incubating the sections without primary antibody - Internal positive control: immunoreactivity of the pancreatic islets Tonini 200541 Whole section Goat polyclonal anti-COX-2 (Santa Cruz Biotechnologies, Santa Cruz, CA, USA), dilution not specified Streptavidin–biotin–peroxidase system - COX-2 (+) = score ≥+1 (positive staining) - Negative control: sections with rabbit or goat preimmune serum Hang 201629 Tissue Microarray Anti-COX-2 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified NS - COX-2 or Sp1 (+) = score ≥ 4 NS - Score = staining intensity (0-3) x % of cells stained (1-4) Anti-Sp1 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified Expressing:Article Title: Prognostic Value of COX-2, NF-κB, and Sp1 Tissue Expressions in Pancreatic Ductal Adenocarcinoma: A Systematic Review and Meta-analysis Article Snippet: General Study Characteristics table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Study Pathologic Specimen Primary Antibody Detection Method Scoring System Controls Fagman 201923 Whole section Polyclonal rabbit antiCOX2 (cytosolic detection), ab15191, Abcam, Cambridge, UK, dilution 1 : 200 Biotin-free polymer-based - COX-2 high grade = >median score Negative control = sections stained by mouse IgG1 (X0931, Agilent Technologies) and rabbit IgG1 (X0903, Agilent Technologies) diluted in 5% non-fat milk and TBS to the same protein concentration as the primary antibody - Score = average intensity score x average percentage of tumor cells showing positive staining Juuti 20065 Whole section Mouse antihuman anti-COX-2 monoclonal antibody (160112;5 Cayman Chemical, Ann Arbor, Michigan, USA) dilution 1 : 200 Avidin–biotin–peroxidase system COX-2 (+) = >5% cells stained with moderate intensity - External positive control: a colon sample containing adenocarcinoma cells stained >50%, and adjacent epithelial non-neoplastic cells stained 5-0% - Internal positive control: pancreatic islet cells that consistently expressed COX-2 - Negative control: Sections with phosphate buffered saline or nonimmune antibody Matsubayashi 20076 Tissue Microarray Anti-COX-2 monoclonal antibody (Cayman Chemical, Ann Arbor, MI) dilution 1 : 100 NS COX-2 (+) = staining intensity was moderate (++) to strong (+++) and the extent was ≥10% in the neighboring fibroblasts NS Pomianowska 201413 Whole Section Monoclonal anti-COX-2 antibodies (Thermo Fischer Scientific rabbit), dilution not specified Biotin-free polymer-based - COX-2 (+) = immunoscore >1.4 - Positive control = Islets of Langerhans and duodenum mucosa - Negative control = identical sections without the primary antibody - Immunoscore = the average numeric scores of 5 high-power fields from each case to be considered positive in intensity scoring. .. - 1 = < 10% cells stained, 2 = 10-50% and 3 > 50% Schmid 201340 Whole section Mouse monoclonal anti-COX-2 antibody (clone CX220; Cayman Chemical, Ann Arbor, Mich) dilution 1 : 200 Streptavidin–biotin–peroxidase system - COX-2 (+) = detectable staining - Negative Control: incubating the sections without primary antibody - Internal positive control: immunoreactivity of the pancreatic islets Tonini 200541 Whole section Goat polyclonal anti-COX-2 (Santa Cruz Biotechnologies, Santa Cruz, CA, USA), dilution not specified Streptavidin–biotin–peroxidase system - COX-2 (+) = score ≥+1 (positive staining) - Negative control: sections with rabbit or goat preimmune serum Hang 201629 Tissue Microarray Anti-COX-2 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified NS - COX-2 or Sp1 (+) = score ≥ 4 NS - Score = staining intensity (0-3) x % of cells stained (1-4) Anti-Sp1 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified Avidin-Biotin Assay:Article Title: Prognostic Value of COX-2, NF-κB, and Sp1 Tissue Expressions in Pancreatic Ductal Adenocarcinoma: A Systematic Review and Meta-analysis Article Snippet: General Study Characteristics table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Study Pathologic Specimen Primary Antibody Detection Method Scoring System Controls Fagman 201923 Whole section Polyclonal rabbit antiCOX2 (cytosolic detection), ab15191, Abcam, Cambridge, UK, dilution 1 : 200 Biotin-free polymer-based - COX-2 high grade = >median score Negative control = sections stained by mouse IgG1 (X0931, Agilent Technologies) and rabbit IgG1 (X0903, Agilent Technologies) diluted in 5% non-fat milk and TBS to the same protein concentration as the primary antibody - Score = average intensity score x average percentage of tumor cells showing positive staining Juuti 20065 Whole section Mouse antihuman anti-COX-2 monoclonal antibody (160112;5 Cayman Chemical, Ann Arbor, Michigan, USA) dilution 1 : 200 Avidin–biotin–peroxidase system COX-2 (+) = >5% cells stained with moderate intensity - External positive control: a colon sample containing adenocarcinoma cells stained >50%, and adjacent epithelial non-neoplastic cells stained 5-0% - Internal positive control: pancreatic islet cells that consistently expressed COX-2 - Negative control: Sections with phosphate buffered saline or nonimmune antibody Matsubayashi 20076 Tissue Microarray Anti-COX-2 monoclonal antibody (Cayman Chemical, Ann Arbor, MI) dilution 1 : 100 NS COX-2 (+) = staining intensity was moderate (++) to strong (+++) and the extent was ≥10% in the neighboring fibroblasts NS Pomianowska 201413 Whole Section Monoclonal anti-COX-2 antibodies (Thermo Fischer Scientific rabbit), dilution not specified Biotin-free polymer-based - COX-2 (+) = immunoscore >1.4 - Positive control = Islets of Langerhans and duodenum mucosa - Negative control = identical sections without the primary antibody - Immunoscore = the average numeric scores of 5 high-power fields from each case to be considered positive in intensity scoring. .. - 1 = < 10% cells stained, 2 = 10-50% and 3 > 50% Schmid 201340 Whole section Mouse monoclonal anti-COX-2 antibody (clone CX220; Cayman Chemical, Ann Arbor, Mich) dilution 1 : 200 Streptavidin–biotin–peroxidase system - COX-2 (+) = detectable staining - Negative Control: incubating the sections without primary antibody - Internal positive control: immunoreactivity of the pancreatic islets Tonini 200541 Whole section Goat polyclonal anti-COX-2 (Santa Cruz Biotechnologies, Santa Cruz, CA, USA), dilution not specified Streptavidin–biotin–peroxidase system - COX-2 (+) = score ≥+1 (positive staining) - Negative control: sections with rabbit or goat preimmune serum Hang 201629 Tissue Microarray Anti-COX-2 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified NS - COX-2 or Sp1 (+) = score ≥ 4 NS - Score = staining intensity (0-3) x % of cells stained (1-4) Anti-Sp1 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified |